The role of perlecan in remodeling the extracellular matrix of the liver, lungs and spleen of mice after administration of BCG vaccine and the liposome-encapsulated dextrazide

Despite significant advances in the diagnosis of tuberculosis, issues mechanism development and treatment fibrotic manifestations are relevant take place treatment. The aim study was to content perlecane find out its participation remodeling extracellular matrix organs mice with tuberculous inflammation when they were injected an antitubercular composition. Materials methods. experiment carried on male BALB/c line, which divided into 4 groups 5 individuals each. All animals, exception intact (group 1), retroorbitally BCG vaccine once (0.5 mg microbial bodies 0.2 ml 0.9% NaCl solution) simulate a generalized tuberculosis process. After infection 6 mo for 3 mo, group 2 intraperitoneally 50 µl solution, - liposome-encapsulated dextrazide solution (LEDZ, isoniazid conjugate oxidized dextran enclosed liposomes), LEDZ inhaled by spraying chamber through nebulizer min at rate per animal. last administration removed from experiment, taken, tissue homogenates prepared. perlecane, hyaluronan, protein-bound hydroxyproline, metalloproteinase inhibitors (TIMP-1, TIMP-2) activity metalloproteinases (MMP), hyaluronidases, α2-macroglobulin measured. Results. In all infected mice, perlecan, TIMP-1, TIMP-2 increased, MMP, hyaluronidase, activated. Regardless method LEDZ, severity fibrosis decreased due decrease hyaluronan hydroxyproline lungs. Perlecan intraperitoneal is involved spleen, inhalation reflects liver fibrosis. Conclusion. Correlations perlecan components reflect regulation BCG-induced inflammation.